Training Course
“Molecular Detection of Foodborne Diseases”

ICISE, Quy Nhon City, Binh Dinh province, Vietnam
11-12 December, 2022

Foodborne diseases have become more common over time, resulting in major public health problems globally. Foodborne pathogens can be found in a variety of foods and it is critical to detect them in order to ensure a safe food supply and prevent foodborne diseases. The conventional methods used to detect foodborne pathogen are time consuming and laborious. As a result, a variety of methods have been developed for rapid detection of foodborne pathogens as it is required in many food analyses. Rapid detection methods can be categorized into nucleic acid-based, biosensor-based and immunological-based methods and their advantages are generally time-efficient, sensitive, specific and labor-saving. Among these approaches, real-time PCR is considered to be powerful tool for identification and quantification of foodborne pathogens. However, this assay is not suitable for less developed regions with limited access to a real-time PCR instrument that requires skilled professionals. An alternative approach so-called Loop mediated Isothermal Amplification (LAMP) has been recently established for rapid detection of foodborne diseases. This method is more specific, sensitive, cost effective and rapid than real-time PCR assays. Particularly, the assay performance can work directly with crude samples through simple visualization of reaction outcome that is feasible for on-site applications with restricted resources. The developments of rapid detection methods are vital in prevention and treatment of foodborne diseases.

The objective of the training:

• to enhance research capacity for technicians, researchers, students in the field of molecular diagnosis
• to strengthen laboratory diagnostic capacities in Vietnam and abroad
• to provide a chance for building up a network for further potential collaboration

The training covers the following topics:

1. Theoretical training

• Updated situation of foodborne diseases in Vietnam and the world
• Gaps and needs for a reliable diagnosis of foodborne pathogens
• Current molecular diagnostic techniques (real-time PCR, PCR, LAMP. and etc.)
• Loop mediated isothermal amplification (LAMP) and its applications for rapid detection of foodborne pathogens
• Interpretation of the results

2. Practical training

• Real-time PCR tests set-up and analysis of the results
• LAMP assays set up with DNA and crude samples and analysis of the results

3. Wrap-up session

• Final discussion of conclusions, recommendations and follow up activities
• Certificate ceremony

Language: English

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Who can apply?

National and international researchers/lecturers/technicians from academic universities, institutes, industry

How to apply?

Registration form available later!

Deadline for registration: October 30, 2023
Acceptance announcement: November 7, 2023
Number of selected trainees: 30 peoples

Registration fee:

• National participants: 3,000,000 VND
• International participants: 150 USD
• Student/postdoc: 1,500,000 VND

The fee will include the following:

• Accommodation
• Lunch and coffee break
• Dinner
• Training kit

Please transact via the following account:

Account name: Rencontres du Vietnam
Account number: 0051000521912
Bank name: Vietcombank
Branch: Quy Nhon city
Description: TrainingMDMD2023_Name of participant

Further inquiries, please contact: mdmd.icise@gmail.com

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Monday 11 December, 2023
7:30 – 8: 30	Course registration and informal introductions	ICISE, Quy Nhon City
8:45 – 9:15	Welcome address Introduction of course work and practical, group photo	Assoc.Prof.Dr. Nguyen Bao Quoc, Nong Lam University Dr. Tran Thanh Son, ICISE, Quy Nhon City
9.15 – 9:45	Lecture 1: TBA (Salmonelosis diseases)	Dr. Tran Thi Sao Mai National Institute for Food Control , Hanoi, Vietnam
9:45 – 10:15	Lecture 2: TBA (Clostridial diseases)	Dr. Pham Bao Yen University of Sciences, Hanoi VNU, Vietnam
10:15 – 10.45	Coffee break/ Tea break
10.45 – 11.15	Lecture 3: TBA (Campylobacteriosis diseases)	Dr. Phung Van Canh Poultry Research Centre, Vietnam
11:15 – 11:35	Lecture 4: TBA (bioinformatics such as genome-wide analysis, primer design, and etc.)	TBA
11:35 – 11:55	Lecture 5: Advanced molecular diagnostic approaches for detection of foodborne pathogens	Assoc.Prof.Dr. Nguyen Bao Quoc Research Institute for Biotechnology and Environment, Faculty of Biological Sciences Nong Lam University, Ho Chi Minh City
12:00 – 13:30	Lunch break
13:30 -14::30	Laboratory Practice: “Guides for real-time PCR”	Assoc.Prof.Dr. Nguyen Duc Hoang University of Science, VNU-HCMC TBA
14:30 – 16:00	Laboratory Practice: “Real-time PCR based assay to detect foodborne pathogens” Group 1: Detection of Salmonella sp. Group 2: Detection of Clostridium sp. Group 3: Detection of Campylobacter sp.	TBA
16:00 – 16:30	Coffee break/ Tea break
16:30 – 17:30	Laboratory Practice: “Real-time PCR based assay to detect foodborne pathogens” (cont.) Group 1: Detection of Salmonella sp. Group 2: Detection of Clostridium sp. Group 3: Detection of Campylobacter sp.	TBA
17:30 -18:00	Questions and Answers (Q&A)	TBA
18:00	Bus to the hotel
Tuesday, 12 December, 2023
8:30 – 9:15	Group presentation (5 minutes each group) Interpreting the results of real-time PCR assays and troubleshooting ”	Assoc.Prof.Dr. Nguyen Duc Hoang, University of Science, VNU-HCMC TBA
9:15 – 10:00	Laboratory Practice: “ TBA”	TBA
10:00 – 10:30	Coffee break/ Tea break
10:30 – 11:30	Laboratory Practice:” Guide for loop mediated isothermal amplification (LAMP)” Group 1: LAMP based Detection of Salmonella sp. Group 2: LAMP based Detection of Clostridium sp. Group 3: LAMP based Detection of Campylobacter sp.	Mr. Nguyen Mai Nghiep Nong Lam University Assoc.Prof.Dr. Nguyen Bao Quoc Nong Lam University Mr. Dao Duy Dat VINASETS company
11:30 – 12:00	Questions and Answers (Q&A)	Assoc.Prof.Dr. Nguyen Bao Quoc Nong Lam University
12:00 – 13:30	Lunch	ICISE, Quy Nhon City
13:30 – 15:30	Laboratory Practice:” Guide for loop mediated isothermal amplification (LAMP)” (cont.) Group 1: LAMP based Detection of Salmonella sp. Group 2: LAMP based Detection of Clostridium sp. Group 3: LAMP based Detection of Campylobacter sp	Mr. Nguyen Mai Nghiep Nong Lam University Assoc.Prof.Dr. Nguyen Bao Quoc Nong Lam University Mr. Dao Duy Dat VINASETS company
15:30 – 16:00	Coffee break/ Tea break
16:00 – 17:00	Group presentation (4 groups with 15 mins/group for presentation)	All instructors and participants
17:00-17:30	Questions and Answers (Q&A)	Assoc.Prof.Dr. Nguyen Bao Quoc Nong Lam University Mr. Dao Duy Dat VINASETS Company Mr. Nguyen Mai Nghiep Nong Lam University
17:30 -18:00	Closing and certificate ceremony
18:00 – 20:00	GALA dinner
20:00	Bus to the hotel
Wednesday and Thursday, 13-14th December, 2023
All day	International workshop on Molecular Diagnostics in Microbiology and Diseases (MDMD2023)

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Lecturers

Assoc.Prof. Dr. Nguyen Bao Quoc Research Institute for Biotechnology and Environment, Faculty of Biological Sciences Nong Lam University, Ho Chi Minh City, Vietnam India	Assoc.Prof. Dr. Nguyen Duc Hoang University of Sciences, Vietnam National University Ho Chi Minh City, Vietnam	Dr. Tran Thi Sao Mai National Institute of Food Control, Hanoi, Vietnam
Dr. Pham Bao Yen University of Science, Vietnam National University Hanoi, Vietnam	Dr. Phung Van Canh TBA, Hanoi, Vietnam

Trainers

Dr. Vu Thi Hoai Thu Hanoi Medical University, Hanoi, Vietnam